Dunn Research Group
CL Hueschen, AR Dunn, R Phillips. Wildebeest Herds on Rolling Hills: Flocking on Arbitrary Curved Surfaces. bioRxiv 2022.
NA Bax*, A Wang*, DL Huang, S Pokutta, WI Weis, AR Dunn. Multi-level force-dependent allosteric enhancement of αE-catenin binding to F-actin by vinculin. bioRxiv 2022.
Amani A Hariri, Sharon S Newman, Steven Tan, Dan Mamerow, Alexandra M Adams, Nicolò Maganzini, Brian L Zhong, Michael Eisenstein, Alexander R Dunn, H Tom Soh. Improved immunoassay sensitivity and specificity using single-molecule colocalization. Nature Communications 2022.
Jia-Yang Chen, [...], Alexander R Dunn, Curtis W Frank, Yi-Chun Wu, Muh-Hwa Yang, Ying-Chih Chang. Multiomic characterization and drug testing establish circulating tumor cells as an ex vivo tool for personalized medicine. iScience 2022.
A Wang, AR Dunn. Mechanism of the cadherin–catenin F-actin catch bond interaction. eLife 2022.
BL Zhong, VT Vachharajani, AR Dunn. Facile detection of mechanical forces across proteins in cells with STReTCh. Cell Reports Methods 2022.
CM Miller, E Korkmazhan, AR Dunn. Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements. Nature Communications 2022.
E Korkmazhan, AR Dunn. The membrane-actin linker ezrin acts as a sliding anchor. Science Advances 2022.
E Korkmazhan, AS Kennard, C Garzon-Coral, CG Vasquez, AR Dunn. Tether-guided lamellipodia enable rapid wound healing. Biophysical Journal 2022.
LM Owen, NA Bax, WI Weis, AR Dunn. The C-terminal actin-binding domain of talin forms an asymmetric catch bond with F-actin. PNAS 2022.
E Korkmazhan, AR Dunn. High-order correlations in species interactions lead to complex diversity-stability relationships for ecosystem. Physical Review E 2022.
CG Vasquez*, EL de la Serna*, AR Dunn. How cells tell up from down and stick together to construct multicellular tissues – interplay between apicobasal polarity and cell–cell adhesion. Journal of Cell Science 2021.
E Korkmazhan, P Tompa, AR Dunn. The role of ordered cooperative assembly in biomolecular condensates. Nature Reviews Molecular Cell Biology 2021.
CG Vasquez, VT Vachharajani, C Garzon-Coral, AR Dunn. Physical basis for the determination of lumen shape in a simple epithelium. Nature Communications 2021.
L Engel, CG Vasquez, EA Montabana, BM Sow, MP Wakiewicz, WI Weis, A R Dunn. Micropatterning of electron microscopy grids for improved cellular cryo-electron tomography throughput. Journal of Structural Biology 2021.
KM Tharp, R Higuchi-Sanabria, GA Timblin, B Ford, C Garzon-Coral, C Schneider, JM Muncie, C Stashko, JR Daniele, AS Moore, PA Frankino, S Homentcovschi, SS Manoli, H Shao, AL Richards, KH Chen, JT Hoeve, GM Ku, M Hellerstein, DK Nomura, K Saijo, J Gestwicki, AR Dunn, NJ Krogan, DL Swaney, A Dillin, VM Weaver. Adhesion-mediated mechanosignaling forces mitohormesis. Cell Metabolism 2021.
RE Wilson, AK Denisin, AR Dunn, BL Pruitt. 3D Microwell Platforms for Control of Single Cell 3D Geometry and Intracellular Organization. Cellular and Molecular Bioengineering, 1-14. (2020).
Tan, S. J., Chang, A. C., Miller, C. M., Anderson, S. A., Prahl, L. S., Odde, D. J., Dunn, A. R. Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds. Science Advances. May 2020.
Kan, W., Enos, M. D., Korkmazhan, E., Muennich, S., Chen, D. H., Gammons M. V., Vasishtha M., Bienz, M., Dunn A.R., Skiniotis, G., Weis, W.I. Limited Dishevelled/Axin oligomerization determines efficiency of Wnt/β-catenin signal transduction. eLife (2020).
Majzner, R. G., Rietberg, S. P., Sotillo, E., Dong, R., Vachharajani, V. T., Labanieh, L., Myklebust, J. H., Kadapakkam, M., Weber, E. W., Tousley, A. M., Richards, R. M., Heitzeneder, S., Nguyen, S. M., Wiebking, V., Theruvath, J., Lynn, R. C., Xu, P., Dunn, A. R., Vale, R. D., Mackall, C. L. Tuning the Antigen Density Requirement for CAR T Cell Activity. Cancer Discovery (2020).
Broussard, J. A., Jaiganesh, A., Zarkoob, H., Conway, D. E., Dunn, A. R., Espinosa, H. D., Janmey, P. A., Green, K. J. Scaling up single-cell mechanics to multicellular tissues - the role of the intermediate filament-desmosome network. Journal of Cell Science(2020).
Cui, K.W., Engel, L., Dundes C.E., Nguyen, T.C., Loh, K.M., Dunn, A.R. Spatially controlled stem cell differentiation via morphogen gradients: A comparison of static and dynamic microfluidic platforms. J. Vac. Sci. Technol. 38, 033205 (2020).
Michalaki, E., Surya, V.N., Fuller, G.G., Dunn, A. R. Perpendicular alignment of lymphatic endothelial cells in response to spatial gradients in wall shear stress. Commun Biol 3, 57 (2020).
Ellefsen, K. L.; Holt, J. R.; Chang, A. C.; Nourse, J. L.; Arulmoli, J.; Mekhdjian, A. H.; Abuwarda, H.; Tombola, F.; Flanagan, L. A.; Dunn, A. R.; Parker, I.; Pathak, M. M. Myosin-II Mediated Traction Forces Evoke Localized Piezo1-Dependent Ca2+ Flickers. Commun. Biol. 2019, 2 (1), 298.
Bielczyk-Maczyńska, E.; Taylor, B.; Miller, C. M.; Zhao, M. L.; Shah, A.; Bahrami-Nejad, Z.; Dunn, A. R.; Teruel, M. N. Loss of Adipocyte Identity through Synergistic Repression of PPARγ by TGF-β and Mechanical Stress. bioRxiv April 2019, 604231.
Tao, H.; Zhu, M.; Lau, K.; Whitley, O. K. W.; Samani, M.; Xiao, X.; Chen, X. X.; Hahn, N. A.; Liu, W.; Valencia, M.; Wu, M.; Wang, X.; Fenelon, K. D.; Pasiliao, C. C.; Hu, D.; Wu, J.; Spring, S.; Ferguson, J.; Karuna, E. P.; Henkelman, R. M.; Dunn, A.; Huang, H.; Ho, H.-Y. H.; Atit, R.; Goyal, S.; Sun, Y.; Hopyan, S. Oscillatory Cortical Forces Promote Three Dimensional Cell Intercalations That Shape the Murine Mandibular Arch. Nat. Commun. April 2019.
Surya N., Vinay*, Michalaki, E. M.*, Fuller, G. G., Dunn, AR. Lymphatic endothelial cell calcium pulses are sensitive to spatial gradients in wall shear stress. MBoC. March 2019.
Price, A. J., Cost, A. L., Ungewiβ, H., Waschke, J., Dunn, A. R., Grashoff, C. Mechanical loading of desmosomes depends on the magnitude and orientation of external stress. Nat. Commun. 9, 5284 (2018).
Dunn, AR. Mechanobiology: ubiquitous and useful. MBoC. 2018. 29(16), 1917-1918.
Ellefsen, K.; Chang, A.; Holt, J. R.; Nourse, J. L.; Arulmoli, J.; Mekhdjian, A.; Abuwarda, H.; Tombola, F.; Flanagan, L. A.; Dunn, A. R.; Parker, I.; Pathak, M. M. Myosin-II mediated traction forces evoke localized Piezo1 Ca2+ flickers. bioRxiv 2018, 294611.
Huang DL, Bax NA, Buckley CD, Weis WI, Dunn AR. Vinculin forms a directionally asymmetric catch bond with F-actin. Science. 2017;357(6352):703-706.
Owen, L M, Adhikari, A S, Patel, M, Grimmer, P, Leijnse, N, Kim, MC, Notbohm, J, Franck, C, Dunn, AR. A cytoskeletal clutch mediates cellular force transmission in a soft, 3D extracellular matrix. MBoC. June 2017. doi: 10.1091/mbc.E17-02-0102
Chang, A. H.; Raftrey, B. C.; D’Amato, G.; Surya, V. N.; Poduri, A.; Chen, H. I.; Goldstone, A. B.; Woo, J.; Fuller, G. G.; Dunn, A. R.; Red-Horse, K. DACH1 Stimulates Shear Stress-Guided Endothelial Cell Migration and Coronary Artery Growth through the CXCL12-CXCR4 Signaling Axis. Genes Dev. 2017.
Krieg, M.; Stühmer, J.; Cueva, J. G.; Fetter, R.; Spilker, K.; Cremers, D.; Shen, K.; Dunn, A. R.; Goodman, M. B. Genetic Defects in β-Spectrin and Tau Sensitize C. Elegans Axons to Movement-Induced Damage via Torque-Tension Coupling. Elife 2017.
Mekhdjian, Armen H, Kai, FuiBoon, Rubashkin, Matthew G, Prahl, Louis S, Przybyla, Laralynne M, McGregor, Alexandra L ,Bell, Emily S, Barnes, J Matthew, DuFort, Christopher C, Ou, Guanqing, Chang, Alice C, Cassereau, Luke, Tan, Steven J, Pickup, Michael W, Lakins, Jonathan N, Ye, Xin, Davidson, Michael W, Lammerding, Jan, Odde, David J, Dunn, Alexander R, Weaver, Valerie M. Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix. MBoC. March 2017. doi: 10.1091/mbc.E16-09-0654
Price AJ, Huang EY, Sebastiano V, Dunn AR. A semi-interpenetrating network of polyacrylamide and recombinant basement membrane allows pluripotent cell culture in a soft, ligand-rich microenvironment. Biomaterials. 2016;121:179-192. doi:10.1016/j.biomaterials.2016.12.005
Vinay N. Surya, Eleftheria Michalaki, Eva Y. Huang, Gerald G. Fuller, Alexander R. Dunn. Sphingosine 1-phosphate receptor 1 regulates the directional migration of lymphatic endothelial cells in response to fluid shear stress. Journal of Royal Society of Interface. 2016. Volume 13, Issue 125.
Chang AC, Mekhdjian AH, Morimatsu M, Denisin AK, Pruitt BL, Dunn AR. Single Molecule Force Measurements in Living Cells Reveal a Minimally Tensioned Integrin State. ACS Nano. October 2016. doi:10.1021/acsnano.6b03314.
Lockhead D, Schwarz EM, O'Hagan R, Bellotti S, Krieg M, Barr MM, Dunn AR, Sternberg PW, Goodman MB. The tubulin repertoire of C. elegans sensory neurons and its context-dependent role in process outgrowth. Mol Biol Cell. 2016 Sep 21. pii: mbc.E16-06-0473
Sun Z, Tseng HY, Tan S, Senger F, Kurzawa L, Dedden D, Mizuno N, Wasik AA, Thery M, Dunn AR, Fässler R. Kank2 activates talin, reduces force transduction across integrins and induces central adhesion formation. Nat Cell Biol. 2016 Sep;18(9):941-53.
Dunn AR. How Hydra Eats. Biophys J. 2016 Apr 12;110(7):1467-8.
Nakayama KH, Surya VN, Gole M, Walker TW, Yang W, Lai ES, Ostrowski MA, Fuller GG, Dunn AR, Huang NF. Nanoscale Patterning of Extracellular Matrix Alters Endothelial Function under Shear Stress. Nano Letters. 2016 January 13.
Ostrowski, M. A., Huang, E. Y., Surya, V. N., Poplawski, C., Barakat, J. M., Lin, G. L., Dunn, A. R. Multiplexed Fluid Flow Device to Study Cellular Response to Tunable Shear Stress Gradients. Annals of Biomedical Engineering. 2015 November 20.
Chai, J., Hamilton, A., Krieg, M., Buckley, C., Riedel-Kruse, I., Dunn, A. R. A Force Balance Can Explain Local and Global Cell Movements During Early Zebrafish Development. Biophysical Journal. 2015 July 21.
Sim, J. Y., Moeller, J., Hart, K. C., Ramallo, D., Vogel, V., Dunn, A. R., Pruitt, B. L. Spatial distribution of cell-cell and cell-ECM adhesions regulates force balance while main-taining E-cadherin molecular tension in cell pairs. Molecular Biology of the Cell, 26(13), 2456-65. 2015 July 1.
Morimatsu, M., Mekhdjian, A. H., Chang, A. C.-C., Tan, S. J., & Dunn, A. R. Visualizing the interior architecture of focal adhesions with high-resolution traction maps. Nano Letters. 2015 March.
Price, A., Dunn, AR. Energetics and Forces in Living Cells. Physics Today. 2015 February.
Pruitt, B. L., Dunn, AR, Weis, W. I., Nelson, W. J. Mechano-Transduction: From Molecules to Tissues. PloS Biology. 2014 November 18.
Buckley, Craig D., Tan, Jiongyi, Anderson, Karen L., Hanein, Dorit, Volkmann, Niels, Weis, William I., Nelson, W. James,Dunn AR. The minimal cadherin-catenin complex bind to actin filaments under force. Science. 2014 October 31.
Kreig M, Dunn AR, Goodman MB. Mechanical Control of the sense of touch by ß-spectrin. Nature Cell Biology. 2014 February 23.
Ostrowski MA, Huang NF, Walker TW, Verwijlen T, Poplawski C, Khoo AS, Cooke JP, Fuller GG, Dunn AR. Microvascular endothelial cells migrate upstream and align against the shear stress field created by impinging flow. Biophysical Journal. 2014 January 21.
Morimatsu M, Mekhdjian AH, Adhikari AS, Dunn AR. Molecular tension sensors report forces generated by single integrin molecules in living cells. Nano Letters. 2013 July 16.
Borghi N, Sorokina M, Shcherbakova OG, Weis WI, Pruitt BL, Nelson WJ, Dunn AR. E-cadherin is under constitutive actomyosin-generated tension that is increased at cell-cell contacts upon externally applied stretch. PNAS. 2012 July 16.
Adhikari AS, Glassey E, Dunn AR. Conformational Dynamics Accompanying the Proteolytic Degradation of Trimeric Collagen I by Collagenases. JACS. 2012 June 21.
Adhikari AS, Chai J,Dunn AR. Multiplexed Single-molecule Force Proteolysis Measurements Using Magnetic Tweezers. JoVE. 2012.
Adhikari AS, Mekhdjian AH,Dunn AR. Strain Tunes Proteolytic Degradation and Diffusive Transport in Fibrin Networks. Biomacromolecules. 2011 Dec 20.
Adhikari A, Chai J,Dunn AR. Mechanical Load Induced a 100-fold increase in the Rate of Collagen Proteolysis by MMP-1. JACS. 2011 Jan 19.
Chuan P, Spudich JA,DunnAR. Robust Mechanosensing and Tension Generation by Myosin VI. J Mol Biol. 2010 Oct 21.
Sung J, Sivaramakrishnan S,Dunn AR, Spudich JA. Single-molecule dual-beam optical trap analysis of protein structure and function. Methods Enzymol. 2010;475:321-75.
Dunn AR, Chuan P, Bryant Z, Spudich JA. Contribution of the myosin VI tail domain to processive stepping and intramolecular tension sensing. PNAS. 2010 Apr 12.
Pierobon P, Achouri S, Courty S,Dunn AR, Spudich JA, Dahan M, Cappello G. Velocity, processivity, and individual steps of single myosin V molecules in live cells. Biophys. J. 2009. 96(10):4268
Dunn AR; Spudich, J. A. Single-molecule gold-nanoparticle tracking with high temporal and spatial resolution and without an applied load. Laboratory Manual for Single Molecule Studies, Cold Spring Harbor Laboratory Press, Woodbury, NY; 2007
Tang, S., Liao, J.-C.,Dunn, A. R., Altman, R. B., Spudich, J. A. Schmidt, J. P. Predicting allosteric communication in myosin via a pathway of conserved residues. J. Mol. Biol. 373, 1361-1373 (2007).
Dunn, A. R. Spudich, J. A. Dynamics of the unbound head during myosin V processive translocation. Nat. Struct. Mol. Biol. 14,246-248 (2007).
Rock, R. S., Ramamurthy, B.,Dunn, A. R., Beccafico, S., Rami, B. R., Morris, C., Spink, B. J., Franzini-Armstrong, C., Spudich, J. A. Sweeney, H. L. A flexible domain is essential for the large step size and processivity of myosin VI. Mol. Cell 17, 603-609 (2005).
Contakes, S. M., Juda, G. A., Langley, D. B., Halpern-Manners, N. W., Duff, A. P.,Dunn, A. R., Gray, H. B., Dooley, D. M., Guss, J. M. Freeman, H. C. Reversible inhibition of copper amine oxidase activity by channel-blocking ruthenium(II) and rhenium(I) molecular wires. Proc. Natl. Acad. Sci. U.S.A. 102, 13451-12456 (2005).
Belliston-Bittner, W.,Dunn, A. R., Nguyen, Y. H. L., Stuehr, D. J., Winkler, J. R. Gray, H. B. Picosecond photoreduction of inducible nitric oxide synthase by rhenium(I)-diimine wires. J. Am. Chem. Soc. 127, 15907-15915 (2005).
Dunn, A. R., Belliston-Bittner, W., Winkler, J. R., Getzoff, E. D., Stuehr, D. J. Gray, H. B. Luminescent ruthenium(II)- and rhenium(I)-diimine wires bind nitric oxide synthase. J. Am. Chem. Soc. 127, 5169-5173 (2005).
Rucker, V. C.,Dunn, A. R., Sharma, S., Dervan, P. B. Gray, H. B. Mechanism of sequence-specific fluorescent detection of DNA by N-methyl-imidazole, N-methyl-pyrrole, and ß-alanine linked polyamides. J. Phys. Chem. B 108, 7490-7494 (2004).
Hays, A.-M. A.,Dunn, A. R., Chiu, R., Gray, H. B., Stout, C. D., Goodin, D. B. Conformational states of cytochrome P450cam revealed by trapping of synthetic molecular wires. J. Mol. Biol. 2, 455-469 (2004).
Dunn, A. R., Dmochowski, I. J., Winkler, J. R. Gray, H. B. Nanosecond photoreduction of cytochrome P450cam by channel-specific electron tunneling Ru-diimine wires. J. Am. Chem. Soc. 41, 12450-12456 (2003).
Dunn, A. R., Hays, A.-M. A., Goodin, D. B., Stout, C. D., Chiu, R., Winkler, J. R. Gray, H. B. Fluorescent probes for cytochrome P450 structural characterization and inhibitor screening. J. Am. Chem. Soc. 124, 10254-10255 (2002).
Dmochowski, I. J.,Dunn, A. R., Wilker, J. J., Crane, B. R., Green, M., Dawson, J. H., Sligar, S. G., Winkler, J. R. Gray, H. B. Ruthenium probes of P450 structure and mechanism. Meth. Enzymol. 357, 120-133 (2002).
Dunn, A. R., Dmochowski, I. J., Bilwes, A. M., Gray, H. B. Crane B. R. Probing the open state of cytochrome P450cam with ruthenium-linker substrates. Proc. Natl. Acad. Sci. U.S.A. 98, 12420-12425 (2001).
Weck, M.,Dunn, A. R., Matsumoto, K., Coates, G. W., Lobkovsky, E. B. Grubbs R. H. Influence of perfluoroarene-arene interactions on the phase behavior of liquid crystalline and polymeric materials. Angew. Chem. Int. Ed. Engl. 38, 2741-2745 (1999).
Coates, G. W.,Dunn, A. R., Henling, L. M., Ziller, J. W., Lobkovsky, E. B. Grubbs, R. H. Phenyl-perfluorophenyl stacking interactions: Topochemical[2+2] photodimerization and photopolymerization of olefinic compounds. J. Am. Chem. Soc. 120, 3641-3649 (1998).
Bloom, C. R., Wu, N.,Dunn, A., Kaarsholm, N. C. Dunn, M. F. Comparison of the allosteric properties of the Co(II)- and Zn(II)-substituted insulin hexamers. Biochemistry 37, 10937-10944 (1998).
Coates, G. W.,Dunn, A. R., Henling, L. M., Dougherty, D. A. Grubbs, R. H. Phenyl-perfluorophenyl stacking interactions: A new strategy for supermolecule construction. Angew. Chem. Int. Ed. Engl. 36, 248-251 (1997).